Fig. 3. Western blot analysis of RyR1 and signaling molecules in rat LAT and GAS muscles. Fold changes of pRyR1Ser²⁸⁴⁰ in LAT (A) and GAS (B) muscle upon 1xLevel and 1xDownhill compared to CON conditions show significantly elevated levels after acute exercise stimulations. Fold changes of pCaMKIIThr²⁸⁶ levels in LAT (C) and GAS (D) compared to CON show increased levels after acute exercise stimulations. Fold changes of pAktSer⁴⁷³ levels in LAT (E) and GAS (F) compared to CON. Fold changes of pPTENSer³⁸⁰ levels in LAT (G) and GAS (H) compared to CON show increased levels after acute exercise stimulations. Representative western blots of pRyR1Ser²⁸⁴⁰, total RyR1, pCaMKIIThr²⁸⁶, pAktSer⁴⁷³, PI3K, and pPTENSer³⁸⁰ as well as the internal loading control GAPDH are depicted in (I). * p<0.05, **p<0.01. n(western blot experiments) = 8 for each tested condition.